Microbial identification and phenotyping systems

Identification of bacteria, yeast and fungi

Biolog microbial identification system allows you to rapidly and accurately identify over 2,500 species of aerobic and anaerobic bacteria, yeast, and fungi. These easy to use systems provide reference laboratory quality identifications.  Biolog systems do this without the labour-intensive requirements of conventional strips or panels. There is a Biolog system for any size laboratory, from full and semi-automatic to manually read systems. The new generation redox chemistry enables testing and identifying both gram-negative and gram-positive bacteria on the same test panel. Gram stain and other pre-tests not needed. Simple one-minute set-up procedure. The expanded GEN III database is designed to meet the needs of Biolog’s broad customer base covering diverse disciplines of microbiology.

Microbial identification is based on the metabolic fingerprint principle. Biolog system is designed for microorganism identification based on species-specific metabolic fingerprints using the differential metabolism observed on the 96-well microplates with 94 different carbon sources and chemical sensitivity assays. Different microorganisms utilise different sets of carbon sources by producing a unique phenotypic fingerprint. There is increased respiration in the microplate wells, where cells can utilise a carbon source. Increased microbial respiration causes reduction of the tetrazolium redox dye, forming a purple colour of the microplate well. The final result is the combination of purple and colourless wells, which is characteristic to a specific microorganism. Then the combination is read with the microplate reading software and the microorganism is identified at a species level in a few seconds.

Biolog Phenotype MicroArrays (PMs) technology is designed to determine the effect of environmental changes on cell's metabolic changes.