The advantage of reverse phase chromatography is that it delivers high levels of purity in a rapid and economical manner. Preparative column chromatography plays an important role in purifying valuable compounds in research or production. If silica gel for advanced normal-phase and reversed phase chromatography is required, we recommend using the irregular shaped LiChroprep® or the perfect spherical PharmPrep™ - highly versatile materials providing fast, effective and reproducible separation.For reverse phase chromatography the quality of sorbent is of tremendous importance. One of the most important factors in chromatography is selectivity. It is decisive in determining the quality and the reproducibility of a chromatographic separation. Merck's inorganic sorbents will deliver reproducible results within narrow band ranges. This provides the highest degree of reliability for chromatographic separations and purifications in the laboratory, pilot plant and production.
Protein purification and final cleaning depends on chosen chromatography techniques that isolate targeted proteins and remove all remaining impurities. This method is based on differing hydrophobic interaction of particles of different molecular mass between the stationary and mobile phase of the chromatography column.
Hydrophobic interaction chromatography is a perfect choice for protein purification. This technique is based on a hydrophobic interaction between hydrophobic ligands and hydrophobic groups of the protein molecules. High-concentration salt buffer helps to separate the needed protein in a more efficient manner.Molecular sieve chromatography (gel filtration) is designed for final protein purification. The requirements to the buffer are quite simple. Native or recombinant proteins, viruses and plasma of biological origin can be easily purified with molecular sieve chromatography columns.
It is paramount to ensure stability and cleanness in each step of every production process. That is why we offer a wide range of chemical substances, from salts to concentrated solutions. All preparations are ready-to-use and packed in single-use packs or rigid plastic containers. Merck EMPROVE® bio and EMPROVE® exp products are provided with an extensive quality assurance documentation. EMPROVE® products will make help you to work faster and more cost-efficient.
Buffer is used in nearly each stage of any biopharmaceutical manufacturing process, which is why buffer quality impacts the success of the process. Merck offers high-quality buffer substances, compatible with all commonly used buffer systems, such as phosphates, acetates, and organic buffers TRIS, HEPES, MES.
Production processes are developed to obtain reduced microbiological and endotoxin contamination. Moreover, enzyme activity (DNase, RNase, protease) of most buffers is still unknown, but crucial for biochromatography. To this effect qualification kits with samples from three batches can be used.Vast amounts of cleaning-in-place (CIP) substances are used in the biopharmaceutical manufacturing. Some manufacturers order on-demand made cleaning substances. Merck has developed high-quality solutions for cleaning and storing of chromatography media. These are Fractogel® and ProSep® sorbents and Pellicon® cassettes. All products fulfil the highest requirements to purity and reliability, and effectively prevent contamination of your valuable purification equipment. All solutions are manufactured under GMP conditions.