In HPLC, components of a mixture are carried through the stationary phase by the flow of a mobile phase and separation is based on differences in migration rates among the sample components. Therefore, the nature of your analytes defines not only the method but also the HPLC system.
Main characteristics of the analytes:
MOLECULAR WEIGHT defines the pore size of the stationary phase
SOLUBILITY defines the HPLC mode, chemistry of stationary phase and eluent.
CONCENTRATION AND MATRIX define the detection parameters and column dimensions.The solubility of your analytes defines the HPLC mode. The elutropic series defines the solvent strength for the most often used chromatography modes normal phase and reversed phase.
The analytical HPLC and UHPLC systems of the KNAUER AZURA liquid chromatography instruments are designed to support and facilitate your work. Whether doing routine analysis or demanding separation tasks, AZURA systems are the right tool to overcome your analytical challenges. Choose between different gradient forming technologies and maximum flow rates to find the best configuration for your task.
The general objective of preparative chromatography is to isolate, purify and collect your target compounds. Preparative applications are often initially performed on an analytical level and need to be upscaled. Depending on the desired scale, the requirements for a preparative system differ in eluent supply, sample injection, column, and detection.