Chromatography equipment

In HPLC, components of a mixture are carried through the stationary phase by the flow of a mobile phase and separation is based on differences in migration rates among the sample components. Therefore, the nature of your analytes defines not only the method but also the HPLC system.

Main characteristics of the analytes:

MOLECULAR WEIGHT defines the pore size of the stationary phase

SOLUBILITY defines the HPLC mode, chemistry of stationary phase and eluent.

CONCENTRATION AND MATRIX define the detection parameters and column dimensions. 

The solubility of your analytes defines the HPLC mode. The elutropic series defines the solvent strength for the most often used chromatography modes normal phase and reversed phase.

Analytical chromatography

The analytical HPLC and UHPLC systems of the KNAUER AZURA liquid chromatography instruments are designed to support and facilitate your work. Whether doing routine analysis or demanding separation tasks, AZURA systems are the right tool to overcome your analytical challenges. Choose between different gradient forming technologies and maximum flow rates to find the best configuration for your task.

Preparative HPLC systems

The general objective of preparative chromatography is to isolate, purify and collect your target compounds. Preparative applications are often initially performed on an analytical level and need to be upscaled. Depending on the desired scale, the requirements for a preparative system differ in eluent supply, sample injection, column, and detection.

Simulated Moving Bed (SMB) chromatography

Simulated moving bed chromatography (SMBC) is increasingly applied as a separation technique in the pharmaceutical industry, production of fine chemicals, and in the field of bioengineering.

SMB is a method in process chromatography that enables substance mixtures to be continuously separated and extracted in two fractions. Its efficiency is significantly higher than batch chromatography, through better utilization of the column stationary phase.

The SMB process enables the separation of binary mixtures by means of a simulated countercurrent between the solid and liquid phases. This is accomplished with a series of chromatography columns arranged in a ring. An eluent flow circulates through this ring. Two inlets (for feed and eluent) and two outlets (extract/red and raffinate/blue) define four separation zones. By continuously feeding sample and synchronously switching the columns against the eluent flow direction, a countercurrent is achieved between the solid and liquid phases, leading to high purity of both target fractions.

SMB Pilot System for continuous cannabinoid production:

Solutions for lipid nanoparticles production in the pharmaceutical industry

KNAUER, mostly known for its liquid chromatography systems, also supplies production equipment for pharmaceutical lipid nanoparticles. This development was made possible by combining the company’s expertise in high pressure dosing and in laboratory systems engineering. 

New and promising developments in medicine make pharmaceuticals and their active ingredients increasingly complex and bring new challenges. Oligonucleotides, for example, are very susceptible to degradation in the human body. LNPs have shown that they can form a stable basis for the administration of active ingredients like RNA, mRNA, siRNA, or DNA-based APIs. These types of APIs and optimization of delivery to the target cells are a very vivid field of research. 

1) What is the purpose of a Lipid Nanoparticle system?

A Lipid Nanoparticle (LNP) system is used to encapsulate mRNA with lipids. The lipid protection around the mRNA is needed to protect it from degradation and to increase the integration in the body cells.

2) Which functionalities are important for Lipid Nanoparticle (LNP) Production ?

The most important functionalities for high quality Lipid Nanoparticle (LNP) production systems are a very precise pumping technology, fast and reliable switching of liquids and a constant control of parameters via software and flowmeter. I addition, an elaborate mixing technology is required for high quality LNP systems.

Lipid nanoparticles (LNP) for mRNA-based vaccines

KNAUER’s jet mixing technology has demonstrated outstanding performance for small and large-scale production of vaccine lipid nanoparticles. KNAUER supplies small desktop devices to users in research and development as well as complete LNP skids for the pharmaceutical industry.

The systems for production, also called IJM skids (impingement jet mixing), are designed and optimized to meet customer's specifications and documentation requirements. System installations in clean rooms (class c) have been successfully carried out. The systems are equipped with all necessary interfaces for the integration into customer's own PLC (programmable logic controller) systems.

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