HPLC Columns and Stationary phases (bulk sorbents)

High Performance Liquid Chromatography (HPLC) and Ultra High Performance Liquid Chromatography (UHPLC) use liquid mobile phases to transport the sample components through a column packed with a solid material; the stationary phase. The "mode" of chromatography is usually defined by a combination of a certain stationary phase type with a certain mobile phase type. In order to generate a chromatogram that is suitable for qualitative or quantitative purposes, bonded phase chemistry, column dimensions, and particle and pore sizes are parameters that need to be optimized.

TSKgel Chromatography Columns

Tosoh Bioscience offers pre-packed columns and bulk resins under the TSKgel®, TOYOPEARL® and ToyoScreen® brand names. Continuing with a tradition of excellence that began with the introduction of one of the first SEC columns in 1976, we now offer a dedicated system for GPC (gel permeation chromatography) analysis, the EcoSEC GPC System.

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TSKgel UHPLC and HPLC columns are the leading columns for the separation of biomolecules in the biopharmaceutical industry. Applications range from characterization of potential targets in R&D to QC analysis of protein therapeutics.

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Tosoh Bioscience offers a comprehensive line of TSKgel (U)HPLC columns for use with conventional and UHPLC systems to meet your purification needs. The TSKgel product line include columns in nearly every mode of HPLC, including Size Exclusion, Ion Exchange, Reversed Phase, Hydrophobic Interaction, Affinity, and Normal Phase/HILIC.

Click on each mode listed below for a short tutorial on the mode, including an animated video, as well as the variety of TSKgel columns offered in that mode.

 Chromatography mode 

 Types of Compounds analyzed 

Size Exclusion Chromatography (SEC) »

Natural (biopolymers), synthetic polymers

Ion Exchange Chromatography (IEC) »

Charged compounds

Reversed Phase Chromatography (RPC) »

Relatively hydrophobic compounds,
neutrals, weak acids, weak bases

Hydrophobic Interaction Chromatography (HIC) »

Hydrophobic biomolecules

Hydrophilic Interaction Chromatography (HILIC) »

Highly polar compounds

Affinity Chromatography(AFC) »

Carbohydrates, proteins, specific anitgens